Transcription activation by FNR: evidence for a functional activating region 2.

Bacteriol . Green Timo Blake , Anne Barnard , Stephen J . W . Busby and Jeffrey for a Functional Activating Region 2 Transcription Activation by FNR : Evidence

Transcription activation by Escherichia coli FNR protein: similarities to, and differences from, the CRP paradigm.

During transcription activation at FNR-dependent promoters where the DNA site for FNR overlaps the -35 element, a surface-exposed activating region in the upstream subunit of the FNR dimer interacts with the C-terminal domain of the RNA polymerase alpha subunit. Starting with a cloned fnr gene encoding a defective FNR derivative carrying substitutions in this activating region, we screened a li...

Spacing requirements for transcription activation by Escherichia coli FNR protein.

We cloned a consensus DNA site for the Escherichia coli FNR protein at different locations upstream of the E. coli melR promoter. FNR can activate transcription initiation at the melR promoter when the FNR binding site is centered around 41, 61, 71, 82, and 92 bp upstream from the transcription start. The SF73 positive control amino acid substitution in FNR interfered with transcription activat...

Additional determinants within Escherichia coli FNR activating region 1 and RNA polymerase alpha subunit required for transcription activation.

The global anaerobic regulator FNR is a DNA binding protein that activates transcription of genes required for anaerobic metabolism in Escherichia coli through interactions with RNA polymerase (RNAP). Alanine-scanning mutagenesis of FNR amino acid residues 181 to 193 of FNR was utilized to determine which amino acid side chains are required for transcription of both class II and class I promote...

Contributions of [4Fe-4S]-FNR and integration host factor to fnr transcriptional regulation.

Maintaining appropriate levels of the global regulator FNR is critical to its function as an O(2) sensor. In this study, we examined the mechanisms that control transcription of fnr to increase our understanding of how FNR protein levels are regulated. Under anaerobic conditions, one mechanism that controls fnr expression is negative autoregulation by the active [4Fe-4S] form of FNR. Through DN...